People

Tâm Mignot

Visiting Professor, Collège de France

Presentation

Tâm Mignot is a research director at the CNRS. Since 2006, he has led a team at the Laboratory of Bacterial Chemistry, which he also directed from 2018 to 2026. His work aims to understand how molecular mechanisms—studied at the single-cell level—enable the emergence of complex collective behaviors in bacteria. In particular, his team has developed models describing how predatory bacteria detect their prey, kill them through direct contact, and then feed on them. More recently, his research has focused on the ecological role of these bacteria in soils, where they are ubiquitous. The results suggest that they play a key role in regulating microbial communities. The team is currently developing experimental systems to reconstruct these communities in the laboratory and study predator–prey interactions under conditions similar to their natural environment, with implications for agriculture and soil restoration. His research is interdisciplinary, at the intersection of molecular biology, biophysics, and ecology. A member of the French Academy of Sciences and the European Academy of Microbiology, he has received several honors, including the CNRS Bronze and Silver Medals, as well as grants from the European Research Council (ERC Starting and Advanced Grants).

Selected bibliography

Articles

  • The mechanism of force transmission at bacterial focal adhesion complexes

    Faure LM, Fiche JB, Espinosa L, Ducret A, Anantharaman V, Luciano J, Lhospice S, Islam ST, Tréguier J, Sotes M, Kuru E, Van Nieuwenhze MS, Brun YV, Théodoly O, Aravind L, Nollmann M, Mignot T. The mechanism of force transmission at bacterial focal adhesion complexes. Nature. 2016 Nov 24;539(7630):530-535. doi: 10.1038/nature20121. Epub 2016 Oct 5. PMID: 27749817; PMCID: PMC5465867.
    This article demonstrates that bacterial motility complexes traffic helically and propel cells in a screw-like rotational motion when they become tethered to the substratum, thus forming focal adhesions.

  • A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus

    Guzzo M, Murray SM, Martineau E, Lhospice S, Baronian G, My L, Zhang Y, Espinosa L, Vincentelli R, Bratton BP, Shaevitz JW, Molle V, Howard M, Mignot T. A gated relaxation oscillator mediated by FrzX controls morphogenetic movements in Myxococcus xanthus. Nat Microbiol. 2018 Aug;3(8):948-959. doi: 10.1038/s41564-018-0203-x. Epub 2018 Jul 16. PMID: 30013238.
    This article demonstrates that directional motility is regulated by a signal transduction system that inverts the cell polarity and functions in two modes, as an excitable system when the signal is low and as an oscillatory system when the signal is high. The work identifies the molecular basis of these behaviors.

  • Misic, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities

    Panigrahi S, Murat D, Le Gall A, Martineau E, Goldlust K, Fiche JB, Rombouts S, Nöllmann M, Espinosa L, Mignot T. Misic, a general deep learning-based method for the high-throughput cell segmentation of complex bacterial communities. Elife. 2021 Sep 9;10:e65151. doi: 10.7554/eLife.65151. PMID: 34498586; PMCID: PMC8478410.
    Here we developed the first deep learning method to segment bacterial cells in communities of thousands of cells with high accuracy. This algorithm was key to develop methods to study how single cell motility contributes to multicellular patterns.

  • Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation

    Herrou J, My L, Monteil CL, Bergot M, Jain R, Martinez E, Mignot T. Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation. Nat Commun. 2025 May 13;16(1):4425. doi: 10.1038/s41467-025-58967-0. PMID: 40360469; PMCID: PMC12075869.
    Here we show that predatory bacteria kill their prey cells using a novel type of pilus that becomes assembled upon contact with the prey and uses an arsenal of different tips adjusted to penetrate distinct prey types.

  • Fatty acid metabolism and the oxidative stress response support bacterial predation

    Jain R, Le NH, Bertaux L, Baudry J, Bibette J, Denis Y, Habermann BH, Mignot T. Fatty acid metabolism and the oxidative stress response support bacterial predation. Proc Natl Acad Sci U S A. 2025 Feb 4;122(5):e2420875122. doi: 10.1073/pnas.2420875122. Epub 2025 Jan 27. PMID: 39869799; PMCID: PMC11804543.
    Here, using an evolutionary approach, we explore the metabolism underlying bacterial predation and show that predatory bacteria not only exploit lipids derived from their prey but also experience a significant oxidative burst as a consequence of this metabolic strategy.

  • Saulnier JB, Romanos M, Schrohe J, Cuzin C, Calvez V, Mignot T. The mechanism of spatial pattern transition in motile bacterial collectives. bioRxiv 2024.10.28.620572 doi: https://doi.org/10.1101/2024.10.28.620572.
    Here we demonstrate that the single cell rules determined in article 2 are sufficient to explain very largescale multicellular transitions during predation.