Lecture in English.
Abstract
The evolutionary expansion of the human forebrain required increased production of inhibitory, GABAergic neurons by the ganglionic eminences, yet the mechanisms remain unclear. Using multiome sequencing, spatial transcriptomics, live imaging, electron microscopy, and organoid models, we identify diverse progenitors, including outer radial glia-like cells. The primate medial ganglionic eminence exhibits distinctive cytoarchitecture, with progenitors surrounding interconnected doublecortin-enriched nests (DENs) that contain newborn neurons. A PCDH19-dependent adhesion code contributes to DEN formation, while progenitor-restricted PCDH19 expression correlates with the presence of DENs across species. Thus, expanded progenitor diversity and specialized tissue architecture represent coordinated developmental innovations that support increased production of inhibitory neurons in primates.
